Cleavage stimulation factor

Cleavage stimulatory factor or cleavage stimulation factor (CstF or CStF) is a heterotrimeric protein of about 200 kilodaltons that is involved in the cleavage of the 3' signaling region from a newly synthesized pre-messenger RNA (mRNA) molecule. CstF is recruited by cleavage and polyadenylation specificity factor (CPSF) and assembles into a protein complex on the 3' end to promote the synthesis of a functional polyadenine tail, which results in a mature mRNA molecule ready to be exported from the cell nucleus to the cytosol for translation.

The amount of CstF in a cell is dependent on the phase of the cell cycle, increasing significantly during the transition from G0 phase to S phase.

Genes

Lodish H, Berk A, Matsudaira P, Kaiser CA, Krieger M, Scott MP, Zipursky SL, Darnell J. (2004). Molecular Cell Biology. WH Freeman: New York, NY. 5th ed.
Martincic K, Campbell R, Edwalds-Gilbert G, Souan L, Lotze MT, Milcarek C. (1998). Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition. Proc Natl Acad Sci USA 95(19):11095-100.

Carrier proteins, Nucleoprotein: RNA-binding protein

Butyrate response factor 1 • Fragile X mental retardation protein • Rev • hu paraneoplastic encephalomyelitis antigens • mRNA cleavage and polyadenylation factors (Cleavage and polyadenylation specificity factor, Cleavage stimulation factor) • host factor 1 protein • Aconitase (ACO1, ACO2) • nuclear factor 90 proteins (ILF2) • Poly(A)-binding protein (PABPC1, PABPC3, PABPC4) • polypyrimidine tract-binding protein • ribonucleoprotein • RNA cap-binding protein (Cap binding complex, EIF4E, EIF4G1) • SMN complex proteins (SMN1, SMN2, DDX20)

B bsyn: dna (repl, cycl, reco, repr) · tscr (fact, tcrg, nucl, rnat, rept, ptts) · tltn (risu, pttl, nexn) · dnab, rnab/runp · stru (domn, 1°, 2°, 3°, 4°)

Post-transcriptional modification

Nuclear	Precursor mRNA · 5' cap formation · Polyadenylation (CPSF, CstF, PAP, PAB2, CFI, CFII) · Poly(A)-binding protein RNA splicing: intron/exon · snRNP · spliceosome (minor spliceosome, U1) · alternative splicing · pre-mRNA processing factor (PLRG1, PRPF3, PRPF4, PRPF4B, PRPF6, PRPF8, PRPF18, PRPF19, PRPF31, PRPF38A, PRPF38B, PRPF39, PRPF40A, PRPF40B) RNA editing · Polyuridylation

Cytosolic	5' cap methylation Messenger RNA decapping: DCP1A · DCP1B · DCP2 · DCPS · EDC3 · EDC4

see also disorders of transcription and post transcriptional modification B bsyn: dna (repl, cycl, reco, repr) · tscr (fact, tcrg, nucl, rnat, rept, ptts) · tltn (risu, pttl, nexn) · dnab, rnab/runp · stru (domn, 1°, 2°, 3°, 4°)